Wound Healing, Skin, db/db Mouse
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DOCUMENTS/REFERENCES
BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
Skin wound healing is a complex biological process comprising coagulation, inflammation, migration-proliferation, and remodeling. The mouse excisional wound healing model is commonly used to study the mechanisms of wound healing and cutaneous regeneration and evaluate the therapeutic efficacy. Impaired wound healing can also be evaluated by using a diabetic animal.
ORGANISM
Mouse
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Groups of 6 C57BLKS/J Iar- +Leprdb/+Leprdb male mice weighing 50 ± 10 g are used. Under isoflurane gas anesthesia, the shoulder and back region of each animal is shaved. A sharp punch (ID 12 mm) is used to remove the skin including panniculus carnosus and adherent tissues. The wound area, traced onto the clear plastic sheets on Days 1, 3, 5, 7, 9, 11, 13 and 15 are quantitated by use of an Image −ProPlus (Media Cybernetics). Test article (doses recommended by client) and/or vehicle (20 µL, 0.5% carboxymethylcellulose in PBS, pH 7. 4) is applied topically immediately following injury and once daily thereafter for a total of 14 consecutive days. The wound half-closure time (CT50) is determined. Suggested Testing: n=6/group (study design dependent). Doses may be administered TOP, PO, IV, IP and SC. Additional analyses that can be requested include biomarker analysis (protein and/or mRNA) and histopathology etc.
CRITERIA FOR SIGNIFICANCE
p<0.05 compared to vehicle control is considered significant activity
TURNAROUND TIME
STANDARD
30 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
Inflammation/Allergy
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.
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