Visceral Pain, Acetic Acid-Induced Writhing

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BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
Pain may be induced by the intraperitoneal administration of various irritants (e.g., acetic acid) into the peritoneal cavity of mice resulting in a characteristic writhing response.  Such assays are useful to detect agents with primarily mild to moderate peripheral as well as strong central analgesic activity (e.g., cyclooxygenase inhibitors and opioids, respectively).
ORGANISM
Mouse
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Test substance is administered orally (PO; 100 mg/kg) to a group of 5 male ICR mice weighing 23 ± 3 grams (g) one hour before injection of acetic acid (0.5%, 20 mL/kg, IP). The number of writhes is recorded during the 5 to 10- minute period after acetic acid injection. One-way ANOVA followed by Dunnett's test is applied for comparison between vehicle and treatment groups. p<0.05 is considered significant. Suggested Testing: n=5/group (study design dependent). Analgesic effects assessed at an initial dose of 100 mg/kg. Dosing volume at 10 mL/kg.
CRITERIA FOR SIGNIFICANCE
Reduction of AA-induced writhing. One-way ANOVA followed by Dunnett's test is applied for comparison between vehicle control and treated groups.
TURNAROUND TIME
STANDARD
20 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
CNS/Addiction/Pain
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.

 

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