Seizure, Pro-Metrazole

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BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
Epilepsy is a common neurological disorder characterized by seizures that are caused by episodic abnormal electrical activity in the brain.  In the model described below, it is designed to explore the potential convulsant ability of test compounds challenged with a sub-threshold dose of metrazole in rodents.
ORGANISM
Mouse
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Test substance (generally 3 dose levels) is administered orally (PO) to a group of 10 male ICR mice weighing 23 ± 3 g are employed. At 60 minutes post dosing, a sub-threshold dose of metrazole (70 mg/kg i.p.) is administered. At this dose level, 90-100% of untreated animals exhibit only clonic convulsions; tonic convulsions and mortality are not seen. The animals are placed individually in glass jars and observed over a period of 30 minutes for the presence of clonic convulsions, tonic convulsions and mortality. Fisher’s exact test is applied for comparison between vehicle and treated groups. Differences are considered significant at p<0.05. Suggested Testing: n=10/group (study design dependent). Potential convulsant activity assessed at initial doses of 10, 30, and 100 mg/kg (p.o.). Dosing volume at 10 mL/kg.
CRITERIA FOR SIGNIFICANCE
Fisher’s exact test is applied for comparison between vehicle and treated groups. Differences are considered significant at p<0.05.
TURNAROUND TIME
STANDARD
20 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
CNS/Addiction/Pain
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.

 

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