Renal Function
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OVERVIEW
DOCUMENTS/REFERENCES
BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
In vivo pharmacology assays may serve to extend in vitro results or detect potentially important primary and/or secondary pharmacodynamic activities. Semi-quantitative or quantitative data can be generated on request.
ORGANISM
Rat
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Groups of 5 Wistar male or female overnight-fasted rats weighing 150 ± 20 g are used. Each animal is hydrated (15 mL/kg physiological saline p. o. ) and administered together with test article p. o. or vehicle (10 mL/kg). Urine volume is measured over the ensuing 6 hour period and then centrifuged (x400 g, 10 min); Na⁺ and K⁺ concentrations are assayed using an Automatic Electrolyte Analyzer (CIBA CORNING, Model 644) and expressed as µEq/100 g body weight. A 2-fold increase (200%) of Na⁺ excretion (Natriuresis) or K⁺ excretion (Kaliuresis) or 1.5-fold or more increase (150%) of urine volume output in test versus control animals is considered significant. A 50 percent or more (50%) decrease in Na⁺ excretion (Natriuresis) or K⁺ excretion (Kaliuresis) or urine volume output in test versus control animals is considered significant. Suggested Testing: n=5/group.
CRITERIA FOR SIGNIFICANCE
A ≥ 200% increase of K+ or Na+ excretion, a ≥ 150% increase of urine volume output, or ≥ 50% decrease of K+ or Na+ excretion or urine volume output during a 6-hour period in overnight fasted and hydra
TURNAROUND TIME
STANDARD
20 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
Gastroenterology
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.
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