Renal, Chronic Failure, Remnant Kidney
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OVERVIEW
DOCUMENTS/REFERENCES
BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
In vivo pharmacology assays may serve to extend in vitro results or detect potentially important primary and/or secondary pharmacodynamic activities. Semi-quantitative or quantitative data can be generated on request.
ORGANISM
Rat
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Groups of 5 male Wistar rats weighting 300 ± 20 grams (g) are employed. The animals are anesthetized with pentobarbital sodium (50 mg/kg, IP). Subtotal nephrectomy is started with right nephrectomy followed by ligation of 1 or 2 branches (leaving 1 branch intact) of the left renal artery. Post-operatively, the animals are housed individually under constant temperature. Sham-operated rats (without right nephrectomy and left kidney blood vessel ligation) are controls. Administration of test article and vehicle starts 24 hours after surgery, by oral gavage (PO), twice daily for 4 weeks. The sham-operated rats receive only vehicle. All animals are placed in metabolic cages (1 per cage) each week for 24-hour collection of urine. Measurements include urinary protein , urine volume, and electrolyte excretion (sodium, potassium, and chloride). One-way ANOVA followed by Dunnett's test is applied for comparison between vehicle and treatment groups. Suggested Testing: n=5/group (study design dependent). Activity is considered at p<0.05. Remnant kidney assessed at an initial dose of 100 mg/kg. Dosing volume at 10 mL/kg.
CRITERIA FOR SIGNIFICANCE
Changes in renal function. One-way ANOVA followed by Dunnett's test is applied for comparison between vehicle control and treated groups.
TURNAROUND TIME
STANDARD
50 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
Gastroenterology
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.
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