Renal, Acute Renal Failure, Ischemia/Reperfusion

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BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
In vivo pharmacology assays may serve to extend in vitro results or detect potentially important primary and/or secondary pharmacodynamic activities. Semi-quantitative or quantitative data can be generated on request.
ORGANISM
Rat
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Groups of 8 male Sprague-Dawley (SD) rats weighting 250 to 280 grams (g) are used. The animals receive fluid supplements (0.9% NaCl and 5% dextrose/1:1, 10 mL/kg, PO) prior to surgery. The rats are anaesthetized with pentobarbital (50 mg/kg, IP). The abdominal cavity is exposed via a midline incision and administered with heparin (100 U/kg, IV). Both renal arteries are occluded for 45 minutes by using vascular clamps, while the sham control animals are subjected to identical surgical procedure without occlusion of both renal arteries. After the renal clips are removed, the kidneys are observed for additional 1 minute to ensure color change indicating blood reperfusion. Vehicle or test article is administered intravenously (IV) 30 minutes before renal arteries are occluded. Blood and urine samples are collected at 24, 48 and 72 hours after reperfusion for determination of serum creatinine/BUN/Na+ and urine creatinine/Na+. One-way ANOVA followed by Dunnett's test is applied for comparison between vehicle and treatment groups. Activity is considered at p<0.05. Suggested Testing: n=8/group (study design dependent). Acute kidney injury assessed at an initial dose of 30 mg/kg. Dosing volume at 5 mL/kg.
CRITERIA FOR SIGNIFICANCE
Changes in renal function. One-way ANOVA followed by Dunnett's test is applied for comparison between vehicle control and treated groups.
TURNAROUND TIME
STANDARD
20 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
Gastroenterology
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.

 

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