Osteoarthritis Pain, Monosodium Iodoacetate (MIA)-Induced

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BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
The monosodium iodoacetate-induced osteoarthritic rat has been widely used as a laboratory model for the study of arthritic inflammation and pain. The presence of pain in inflammation models is noted as hyperalgesia (increased response to noxious stimuli) or allodynia (nociceptive response to normally innocuous stimuli). In the model described below, sharp withdrawal of the inflamed hind paw to light mechanical stimuli indicates the presence of allodynia.
ORGANISM
Rat
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
A single intraarticular (IA) injection of monosodium iodoacetate (1 mg/rat) is given for mechanical allodynia induction. Rats are pre-selected (clear presence of allodynia) for experimentation only if the nociceptive response 21 days after chemical induction (pre-treatment) was reduced by 10 g of force relative to the response of the individual paw before chemical injection (pre-induction). Test compound and vehicle are administered orally and the level of allodynia is determined at 60 and 120 minutes after test substance or vehicle administration. One-way ANOVA followed by Dunnett's test is applied for comparison between vehicle and treatment groups. Activity is considered at p<0.05. Significant differences are considered at p<0.05. Suggested Testing: n=8/group (study design dependent). Analgesic activity assessed at an initial dose of 100 mg/kg. Dosing volume at 10 mL/kg.
CRITERIA FOR SIGNIFICANCE
Reduction of MIA-induced allodynia. One-way ANOVA followed by Dunnett's test is applied for comparison between vehicle control and treated groups.
TURNAROUND TIME
STANDARD
60 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
CNS/Addiction/Pain
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.

 

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