L5 Spinal Nerve Ligation

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BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
Tissue damage, inflammation or injury of the nervous system may result in chronic neuropathic pain characterised by increased sensitivity to painful stimuli (hyperalgesia), the perception of innocuous stimuli as painful (allodynia) and spontaneous pain. Neuropathic pain has been described in about 1% of the US population, is often severely debilitating and largely resistant to treatment.  Neuropathic pain (allodynia) induced by spinal nerve ligation is shown by the sharp withdrawal of affected hind paw to light mechanical stimuli.
ORGANISM
Mouse
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Groups of 10 male C57BL/6 mouse weighing 25 ± 2 grams (g) are employed. Allodynia is induced by L5 spinal nerve ligation. Mice are preselected (clear presence of allodynia) for experimentation only if the nociceptive response 14 days after nerve ligation (pre-treatment). Test substance and vehicle are administered orally (PO) to groups of 10 animals one hour before the level of allodynia is again determined (post-treatment). One-way ANOVA followed by Dunnett's test is applied for comparison between vehicle and treatment groups. Activity is considered at p<0.05. Suggested Testing: n=10/group (study design dependent). Analgesic activity assessed at an initial dose of 100 mg/kg. Dosing volume at 10 mL/kg.
CRITERIA FOR SIGNIFICANCE
Reduction of SNL-induced allodynia. ANOVA followed by Dunnett's test is applied for comparison between vehicle control and treated groups.
TURNAROUND TIME
STANDARD
60 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
CNS/Addiction/Pain
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.

 

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