Irritable Bowel Syndrome (IBS), Conditioned Fear Stress-Induced
CONTACT CUSTOMER SERVICE
ADD TO FAVORITES
ADD TO PANEL
OVERVIEW
DOCUMENTS/REFERENCES
BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
Stress-induced abnormal defecation using a rat model of conditioned fear stress (CFS), which is known to be psychological stress model useful for the evaluation of anxiolytic, antidepressant, and anti-IBS agents.
ORGANISM
Rat
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Groups of 6 male Sprague-Dawley (SD) rats weighing 200 ± 20 g are used. Animals are trained twice at 24-hours interval in an electric-shock machine with electrode grid-inlaid floor with a program of 3 sec warning sound followed with 5 sec electric shock and intensive light per minute as a trial with a total of 30 exposures per day. Forty-eight (48) hours later, rats are returned to the electric shock chamber to evoke conditioned fear stress (CFS) with warning sound and light in the absence of electric shock for 30 trials to collect the CFS induced defecation. Vehicle, test articles and loperamide are administered orally (PO) 1 hour before the CFS challenge. The incidence of diarrhoea is noted. The severity of diarrhoea is assessed by using an arbitrary scoring scale ranging from 0 to 2: normal stools (0), soft stools (1) and liquid stools (2). In addition, the weight of stool is also measured in this study.
CRITERIA FOR SIGNIFICANCE
p<0.05 compared to vehicle control is considered significant activity
TURNAROUND TIME
STANDARD
25 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
Gastrointestinal
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
ITEM DOES NOT EXIST
© Copyright 2026, Pharmacology Discovery Services – Privacy Policy | Cookie Notice | Cookie Preferences