Inflammatory Pain, Formalin-Induced, Rat

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BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
The formalin test has been proposed as a chronic pain model sensitive to centrally acting analgesic agents. More recently, sub-plantar administered formalin-induced algesia (paw licking) has been divided into two phases:  an early neuronal central phase (within 5 to 10 minutes) and an inflammatory phase (within 10 to 60 minutes). The assay described below relates primarily to the late inflammatory algesic phase sensitive to both strong central analgesic as well as weaker analgesic/anti-inflammatory agents.
ORGANISM
Rat
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Test substance is administered orally (PO) to groups of 8 male Sprague-Dawley (SD) male rats one hour before subplantar injection of formalin (0.05 mL, 2% solution). The hind paw licking time is recorded during a 0-30 minute period at 5-minute intervals after formalin challenge as a measure of analgesic activity of the test compound. One-way ANOVA followed by Dunnett's test is applied for comparison between vehicle and treatment groups. p<0.05 is considered significant. Suggested Testing: n=8/group (study design dependent). Analgesic activity assessed at an initial dose of 100 mg/kg. Dosing volume at 10 mL/kg.
CRITERIA FOR SIGNIFICANCE
Reduction of formalin-induced inflammatory pain. One-way ANOVA followed by Dunnett's test is applied for comparison between vehicle control and treated groups.
TURNAROUND TIME
STANDARD
20 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
CNS/Addiction/Pain
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.

 

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