Inflammatory Pain, Complete Freund's Adjuvant-Induced, Thermal Hyperalgesia

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BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
The Complete Freund’s adjuvant (CFA)-induced arthritic rat has been widely used as a laboratory model for the study of arthritic inflammation and pain. The presence of pain in inflammation models is noted as hyperalgesia (increased response to noxious stimuli) or allodynia (nociceptive response to normally innocuous stimuli). In the model described below, sharp withdrawal of the inflamed hind paw to thermal stimuli indicates the presence of allodynia.
ORGANISM
Rat
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
CFA (0.1 mL, 0.1% solution) is injected intraplantarly for thermal hyperalgesia induction. Twenty four (24) hours after CFA injection, rats are pre-selected (with clear presence of thermal hyperalgesia) for experimentation only if the latency to withdrawal is less than 75% of baseline. Test substance and vehicle are administered by oral (PO) administration 60 minutes before the level of thermal hyperalgesia is again measured (post-treatment). One-way ANOVA followed by Dunnett's test is applied for comparison between vehicle and treatment groups. Activity is considered at p<0.05. Suggested Testing: n=8/group (study design dependent). Analgesic activity assessed at an initial dose of 100 mg/kg. Dosing volume at 10 mL/kg.
CRITERIA FOR SIGNIFICANCE
Reduction of CFA-induced hyperalgesia. One-way ANOVA followed by Dunnett's test is applied for comparison between vehicle control and treated groups.
TURNAROUND TIME
STANDARD
20 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
CNS/Addiction/Pain
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.

 

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