Inflammatory Pain, Carrageenan-Induced, Mechanical Allodynia

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BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
Carrageenan-induced hind limb rat inflammation has been widely used as a laboratory model for the study of inflammation and pain. The presence of pain in inflammation models is noted as hyperalgesia (increased response to noxious stimuli) or allodynia (nociceptive response to normally innocuous stimuli).  In the model described below, sharp withdrawal of the inflamed hind paw to light mechanical stimuli indicates the presence of allodynia.
ORGANISM
Rat
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Carrageenan (0.1 mL of 1% suspension) is injected intraplantarly for allodynia induction. Rats are pre-selected for experimentation only if the nociceptive response 2 hours after carrageenan injection (pre-treatment) is reduced by 10 g of force relative to the response of the individual paw before carrageenan injection (pre-induction). Test substance and vehicle are administered orally (PO) two hours after carrageenan challenge and the applied pressure is again measured three hours after. One-way ANOVA followed by Dunnett's test is applied for comparison between vehicle and treatment groups. Activity is considered at p<0.05. Suggested Testing: n=8/group (study design dependent). Analgesic activity assessed at an initial dose of 100 mg/kg. Dosing volume at 10 mL/kg.
CRITERIA FOR SIGNIFICANCE
Reduction of carrageenan-induced allodynia. One-way ANOVA followed by Dunnett's test is applied for comparison between vehicle control and treated groups.
TURNAROUND TIME
STANDARD
20 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
CNS/Addiction/Pain
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.

 

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