Inflammatory Bowel Disease (IBD), Oxazolone-Induced Colitis
CONTACT CUSTOMER SERVICE
ADD TO FAVORITES
ADD TO PANEL
OVERVIEW
DOCUMENTS/REFERENCES
BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
Human inflammatory bowel disease (IBD) is a chronic inflammatory condition comprising two major disorders, Crohn's disease and ulcerative colitis. The fundamental symptoms of IBD are abdominal pain, bloody diarrhea and body weight loss. Oxazolone (4-ethoxymethylene-2-phenyl-2-oxazolin-5-one, OXA)-induced colitis model has become to be accepted as a T helper-2 (Th-2) type colitis model for human ulcerative colitis (UC) and is commonly used to study the mechanisms of gastrointestinal inflammation and evaluate the therapeutic efficacy.
ORGANISM
Mouse
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Groups of 6 male BALB/c mice at 8 weeks of age are sensitized by applying oxazolone (150 µL, 3% in acetone/olive oil, 4:1 v/v) to their pre-shaved rostral back on Day 0. The animals are re-sensitized with oxazolone on Day 5. Distal colitis is induced by intracolonic instillation of oxazolone solution (100 µL, 1% in 40% ethanol), after which animals are kept in a vertical position for 30 s to ensure that the solution remains in the colon. Control mice receive 100 µL 0. 9% NaCl alone. Test article, and vehicle (10 mL/kg) are administered by oral gavage 24 (Day 4) and 2 hours before oxazolone challenge, followed by once daily dosing through Day 7 for a total of 4 consecutive days. During the experiment, body weight, fecal occult blood and stool consistencya are recorded daily. On Day 8, mice are euthanized by CO2 asphyxiation and colon length is record and weighed. Additional analyses that can be requested include colitis score, biomarkers (protein and/or mRNA), and histopathology etc.
CRITERIA FOR SIGNIFICANCE
p<0.05 compared to vehicle control is considered significant activity
TURNAROUND TIME
STANDARD
30 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
Inflammation/Allergy
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.
ITEM DOES NOT EXIST
© Copyright 2026, Pharmacology Discovery Services – Privacy Policy | Cookie Notice | Cookie Preferences