Inflammatory Bowel Disease (IBD), DNBS-Induced Colitis
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OVERVIEW
DOCUMENTS/REFERENCES
BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
Human inflammatory bowel disease (IBD) is a chronic inflammatory condition comprise of two major disorders, Crohn's disease and ulcerative colitis. The fundamental symptoms of IBD are abdominal pain, bloody diarrhea and body weight loss. 2, 4-Dinitrobenzenesulfonic acid (DNBS)-induced IBD is commonly used to study the mechanisms of gastrointestinal inflammation and evaluate the therapeutic efficacy.
ORGANISM
Rat
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Groups of 6 male Wistar rats weighing 190 ± 10 g are used. Animals fasted overnight are used. Distal colitis is induced by intra-colonic instillation of DNBS (40 mg in 0.5 mL 30% ethanol). Test article and vehicle (10 mL/kg) are administered orally (PO) 24 and 2 hours before DNBS instillation and then daily for 5 days. The body weight is recorded daily. The stool consistency score and fecal occult blood score are measured from Day 3 to Day 8 after the DNBS challenge (Day 2). On Day 8, each colon is removed, scored and weighed. Colon-to-body weight ratio is then calculated for each animal according to the formula: Colon (g)/BW x 100. The “Net” increase in the ratio of Vehicle-control + DNBS group relative to Vehicle-control group is used as a base for comparison with treated groups and expressed as “Dec. (%)” (Percent decrease). ANOVA followed by Dunnett's test is applied for comparison between vehicle and treated groups. p<0.05 is considered significant.
CRITERIA FOR SIGNIFICANCE
p<0.05 compared to vehicle control is considered significant activity
TURNAROUND TIME
STANDARD
30 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
Inflammation/Allergy
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.
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