Gastrointestinal Motility, Colonic Propulsion, Clonidine
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DOCUMENTS/REFERENCES
BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
Changes of gastrointestinal motility, which are important effect related to the food digestion or drug absorption in the gastrointestinal tract. Clonidine- and loperamide-induced delays in colonic transit are models of atonic and spastic constipation. Colonic propulsion induced by clonidine and loperamide in mice is commonly used to study the mechanisms of intestinal transit with the drug.
ORGANISM
Mouse
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Test article is administered to groups of 10 male ICR mice weighing 35 ± 5 g. Clonidine (30 µg/kg, SC) is administered 30 minutes later and after an additional 30 minutes, a glass bead (about 3 mm in diameter) is inserted into the distal colon 2 cm from anus. The time required to evacuate the inserted glass bead is measured over 90 minutes and used as index of colonic propulsive activity. Suggested Testing: n=10/group (study design dependent). Doses may be administered PO, IV, IP and SC.
CRITERIA FOR SIGNIFICANCE
p<0.05 compared to vehicle control is considered significant activity
TURNAROUND TIME
STANDARD
20 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
Gastroenterology
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.
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