Gastrointestinal Motility, 5-HTP-Induced Diarrhea

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BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
Serotonin (5-HT) is present in the gastrointestinal tract and is probably one of the compounds responsible for diarrhoea in human presenting with carcinoid syndrome. The involvement of 5-HT has been shown to cause abnormalities in intestinal transport and secretion of fluids.
ORGANISM
Mouse
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Test article is administered by oral gavage (PO) to groups of 10 male ICR mice weighing 35 ± 5 g either 60 minutes by oral gavage (PO) or 30 minutes by intraperitoneal injection (IP) before dl-5-HTP (dl-5-Hydroxytryptophan, 10 mg/kg IP). Animals are observed during the ensuing 30 minutes after 5-HTP challenge, and the incidence of diarrhoea is noted. The severity of diarrhoea is assessed by using a scoring system ranging from 0 to 3: normal stools (0), soft stools (1), unformed stools (2), and watery stools (3). In addition, the defecation frequency is also recorded. Doses may be administered PO, IV, IP, and SC. ANOVA followed by Dunnett's test is applied for comparison between vehicle and treatment groups. p<0.05 is considered significant.
CRITERIA FOR SIGNIFICANCE
p<0.05 compared to vehicle control is considered significant activity
TURNAROUND TIME
STANDARD
20 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
Gastroenterology
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.

 

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