Gastric Acidity, Pylorus Ligation
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OVERVIEW
DOCUMENTS/REFERENCES
BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
Peptic ulcer disease is one of the most common gastrointestinal disorders, which characterized by mucosal damage secondary to pepsin and gastric acid secretion. It usually occurs in the stomach and proximal duodenum. Pylorus ligated method is carried out for the screening the antiulcer activity.
ORGANISM
Rat
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Groups of 6 Wistar male or female overnight-fasted rats weighing 250 ± 10 g are used. Under pentobarbital anesthesia (50 mg/kg i.p.), the abdominal cavity is exposed and is ligated just below the pylorus sphincter. Test article and/or vehicle is administered intraduodenally (i. d.) immediately following ligation in a volume of 2 ml/kg body weight. Animals are sacrificed 4 hours later and the gastric contents are collected. After centrifugation, the volume of each sample is measured and acidity is determined by titration with 0. 001N NaOH to pH 7. Suggested Testing: n=6/group (study design dependent). Doses may be administered PO, IV, IP and SC.
CRITERIA FOR SIGNIFICANCE
p<0.05 compared to vehicle control is considered significant activity
TURNAROUND TIME
STANDARD
20 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
Gastroenterology
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.
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