Escherichia coli (CDC & FDA AR Isolate Bank #0055), Peritonitis Model, LD90-100
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OVERVIEW
DOCUMENTS/REFERENCES
BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
This model assesses the antimicrobial efficacy of test articles for protection against a lethal peritoneal infection with Escherichia coli. It may be used to assess efficacy of small molecules, vaccines and biologics.
SPECIES
Escherichia coli
STRAIN COMMON NAME
#0055
ORGANISM
Gram-negative Bacteria
INCUBATION CONDITIONS
Aerobic
NOTABLE ANTIBIOTIC RESISTANCE GENE
NDM-1
GENOMIC ACCESSION NUMBER
SAMN04014896
SOURCE
STRAIN SOURCE
CDC & FDA AR Isolate Bank
STRAIN SOURCE ID
AR Bank #0055
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Groups of 10 ICR (CD-1) derived female mice weighing 22 ± 2 gms are used. Each animal is inoculated with an intraperitoneally administered lethal dose of Escherichia coli (AR# 0055) suspended in 0.5 ml brain-heart infusion broth containing 5% mucin. Test substance and vehicle are administered 1 and 13 hours later (or per the client request) by IP, PO, IV or SC injection. The dose (up to 100 mg/kg) and vehicle (recommended 2% Tween 80 or 0.9% NaCl, 10 ml/kg) are specified by the sponsor. Mortality is recorded daily during the following 7 days. Prevention of mortality in 50 percent or more (≥ 50%) of the animals indicates significant activity.
CRITERIA FOR SIGNIFICANCE
> 50% of survival
TURNAROUND TIME
STANDARD
20 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
Infectious Disease
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.
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