Escherichia coli (ATCC 25922), Thigh Infection Model, CFU Data
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OVERVIEW
DOCUMENTS/REFERENCES
BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
This assay assesses the antimicrobial efficacy of test articles in a thigh muscle infection model. The microbial counts in tissue are measured. Strain ATCC 25922 is an antibiotic susceptible laboratory strain that is frequently used for in vitro MIC testing and efficacy testing in mice.
SPECIES
Escherichia coli
ORGANISM
Gram-negative Bacteria
INCUBATION CONDITIONS
Aerobic
SOURCE
STRAIN SOURCE
American Type Culture Collection (ATCC)
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Groups of 5 neutropenic mice are used. Animals are intramuscularly inoculated with pathogen suspension then test articles or vehicle are administered at time points after inoculation. Doses may be administered IV, SC, PO, IM, IP or by IV infusion. At 24 hr after the first treatment, animals are humanely euthanized and tissue is aseptically removed. Tissue is homogenized and pathogen counts are determined by plating to agar medium. Pathogen counts from treatment groups are compared to vehicle groups and the signficance of an effect is determined.
CRITERIA FOR SIGNIFICANCE
Significant difference is determined at ≥ 2-log or P < 0.05
TURNAROUND TIME
STANDARD
25 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
Infectious Disease
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.
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