Enterococcus faecalis VRE (ATCC 51575), Bacteremia Model (Renal Infection), Kidney, CFU Data

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BIOLOGICAL INFORMATION

BACKGROUND INFORMATION

This assay assesses the antimicrobial efficacy of test articles in a systemic infection model of septicemia. The microbial counts in kidney tissue are measured. Enterococcus faecalis is a commensal bacterium that can cause life-threatening infections (particularly in the hospital setting) such as endocarditis, bacteremia, peritonitis, and urinary tract infections. This model is performed with a VanB producing E. faecalis strain that is resistant to vancomycin.

SPECIES

Enterococcus faecalis

ORGANISM

Gram-positive Bacteria

INCUBATION CONDITIONS

Aerobic

SOURCE

STRAIN SOURCE

American Type Culture Collection (ATCC)

ASSAY INFORMATION

ASSAY TYPE

In Vivo

TESTING INFORMATION

PROCEDURE SUMMARY

Groups of 5 immune competent mice are used. Animals are inoculated intravenously with pathogen suspension then test articles or vehicle are administered at time points after inoculation. Doses may be administered IV, SC, PO, IM, IP or by IV infusion. Animals are humanely euthanized with carbon dioxide asphyxiation at time points and kidneys are aseptically removed. Kidney tissue is homogenized and pathogen counts are determined by plating to agar medium. Pathogen counts from treatment groups are compared to vehicle groups and the signficance of an effect is determined.

CRITERIA FOR SIGNIFICANCE

Significant difference is determined at ≥ 2-log or P < 0.05

TURNAROUND TIME

STANDARD

25 Days

CLINICAL RELEVANCE

THERAPEUTIC AREA

Infectious Disease

ADDITIONAL INFORMATION

BRAND

PDS

TESTING LOCATION

Taiwan - Taipei

OTHER INFORMATION

We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.