Diabetic Pain, Streptozotocin (STZ)-Induced
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BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
Painful peripheral neuropathy is a common secondary complication of diabetes. Streptozotocin (STZ)-induced diabetic rats are the animal model commonly used to investigate the diabetic neuropathy and to evaluate potential therapies. A single dose of STZ (50 mg/kg, IP) is used to induce the experimental diabetes in rats. The presence of STZ-induced pain is noted as hyperalgesia (increased response to
noxious stimuli) or allodynia (nociceptive response to normally innocuous stimuli).
ORGANISM
Rat
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Groups of 10 male Sprague-Dawley (SD) rats weighing 225 - 250 grams (g) are employed. Streptozotocin (STZ; 50 mg/kg) freshly dissolved in 0.1 M citrate buffer (pH 4.5) is injected intraperitoneally (IP) on Day 0. Three days later, diabetes is confirmed by existence of blood glucose >350 mg/dL as measured by glucometer. Animal health is monitored biweekly for 14 days, at which point mechanical allodynia and thermal hyperalgesia are measured and confirmed. The animals are tested prior to study inclusion for mechanical allodynia and thermal hyperalgesia. Test substance and vehicle are administered orally (PO) to groups of 10 animals one hour before the level of mechanical allodynia and thermal hyperalgesia were again determined (post-treatment). One-way ANOVA followed by Dunnett's test is applied for comparison between vehicle and treatment groups. Activity is considered at p<0.05. Suggested Testing: n=10/group (study design dependent). Analgesic activity assessed at an initial dose of 100 mg/kg. Dosing volume at 10 mL/kg.
CRITERIA FOR SIGNIFICANCE
Reduction of diabetes-induced allodynia. One-way ANOVA followed by Dunnett's test is applied for comparison between vehicle control and treated groups.
TURNAROUND TIME
STANDARD
45 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
CNS/Addiction/Pain
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.
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