Dermatitis, Atopic, Mite-Induced
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OVERVIEW
DOCUMENTS/REFERENCES
BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
Atopic dermatitis (AD) is characterized by chronic highly pruritic and relapsing inflammatory skin lesions. It is reported that mite allergen, from Dermatophagoides farinae, induces stable clinical AD-like skin diseases in female NC/Nga mice. Skin changes in NC/Nga mice are evidenced by scratching behavior, followed by the rapid development of erythema, lichenification with edema, and hemorrhage. Histological examinations have revealed hyperplasia and accumulation of eosinophils and mast cells in skin lesions. In addition to these skin changes, NC/Nga mice exhibit elevated levels of total serum IgE. Mite-induced AD model can be applied for evaluating potential antipruritic and anti-inflammatory substances.
ORGANISM
Mouse
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Female 8 to 10-week-old NC/Nga mice are randomly divided into groups of eight each. The Biostir®-AD (120 mg/mouse; house dust mite, from Dermatophagoides farinae) is applied twice a week for three weeks (Days 1, 4, 8, 11, 15, and 18), a total of 720 mg Biostir® per mouse. Test articles and vehicle are administered daily by oral gavage (PO) starting on Day 1 through Day 21. Reference compound, tacrolimus (100 mg/animal, 0.1%), is given topically (TOP) daily starting on Day 1 through Day 21. The dosing regimen may change per client's requests. Scratching assay, ear thickness measurement, and clinical scoring are performed weekly on Days 0 (baseline) and 21. Additional analyses that can be requested include scratching, ear thickness and clinical scoring on Days 7 and 14, ear weight, biomarker analysis (protein and/or mRNA), histopathology, etc. Suggested Testing: n=8/group (study design dependent). Antipuritic effects were assessed at an initial dose of 30 mg/kg (PO). Dosing volume is 10 mL/kg.
CRITERIA FOR SIGNIFICANCE
Reduction of atopic dematitis. One-way ANOVA followed by Dunnett's test is applied for comparison between vehicle control and treated groups.
TURNAROUND TIME
STANDARD
45 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
Inflammation/Allergy
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.
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