Depression, Forced Swim Test (FST), Rat

ITEM 527540

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OVERVIEW

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BIOLOGICAL INFORMATION

BACKGROUND INFORMATION

The forced swim test, also known as the behavioral despair test, is used to test for depression-like behavior in rodents. The test includes placing a rat or mouse inside a cylinder filled with water. The mobility of the animal is measured. In general, ‘floating behavior’ is used as a parameter to analyze ‘hopelessness’ and thus depression-like behavior.Z68BX68D68:X68D68:AF69BX68D68:X68D68:AK68D68:AJ68D68:AK68D68:AQ68BX68D68:X68D68:AS68BX68D68:X68D68:BQ68BX68D68:X68D68:BS68BX68D68:X68D68:BU68BX68D68:X68D68:BW68BX68D68:X68D68:BY68BX68D68:X68D68:CB68BX68D68:X68D68:CC68BX68D68:X68D68:CD68D68:CC68D68:CB68D68:BZ68D68:BY68D68:BX68

ORGANISM

Rat

ASSAY INFORMATION

ASSAY TYPE

In Vivo

TESTING INFORMATION

PROCEDURE SUMMARY

A group of 10 male Sprague-Dawley (SD) rats weighing 200 to 250 grams (g) are used. Around 24 hours before testing, rats experience a “pre-swim”, in which they are placed in a clear plastic cylinder (height: 45 cm, diameter: 21.5 cm) individually, filled to 30 cm with water at 23.5-24.5oC, for 15 minutes. At the end of the pre-swim they are towel-dried and placed in a heated chamber (approx. 30oC) for 15 minutes. On the following day, vehicle and test compound are dosed intraperitoneally 30 minutes before the swim test. Data collection is either by the Timed Method, where animals are timed for inactive behavior (immobility) during a 5-minute swim period, where scoring begins 5 seconds after placing the rats in water and continues at 5-second intervals for duration of 5 minutes. Each rat is scored as either Immobile, Swimming, or Climbing. Recorded mobility must be moderately robust, or with apparent intent to escape, otherwise the animal will be considered immobile. After the 5-minute swim the rats are removed from the water and dried and warmed as above. One-way ANOVA followed by Dunnett’s test is applied for a comparison between vehicle and treated groups. Differences are considered significant at p<0.05.

CRITERIA FOR SIGNIFICANCE

Reduction of the duration of immobility during 5 minutes. One-way ANOVA followed by Dunnett’s test is applied for a comparison between vehicle and treated groups.

TURNAROUND TIME

STANDARD

30 Days

CLINICAL RELEVANCE

THERAPEUTIC AREA

CNS/Addiction/Pain

ADDITIONAL INFORMATION

BRAND

PDS

TESTING LOCATION

Taiwan - Taipei

OTHER INFORMATION

We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.