Depression, Forced Swim Test (FST), Mouse

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BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
The forced swim test, also known as the behavioral despair test, is used to test for depression-like behavior in rodents. The test includes placing a rat or mouse inside a cylinder filled with water. The mobility of the animal is measured. In general, ‘floating behavior’ is used as a parameter to analyze ‘hopelessness’ and thus depression-like behavior.
ORGANISM
Mouse
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Test substance is administered orally (PO) to groups of 8 male ICR mice weighing 23 ± 3 grams (g).  One hour later, animals are placed in a 1 liter (L) volume glass beaker (height: 14.5 cm, diameter: 11 cm) containing 10 cm height water at room temperature (22 to 24oC) for 6 minutes. The duration of animal immobility within the last 4 of the 6 minutes is then recorded.  A mouse is considered immobile when it ceases struggling and remains floating in the water making only those movements necessary to keep its head above water. One-way ANOVA followed by Dunnett’s test is applied for a comparison between vehicle and treated groups. Differences are considered significant at p<0.05. Suggested Testing: n=8/group (study design dependent). Antidepressant activity assessed at an initial dose of 100 mg/kg. Dosing volume at 10 mL/kg.
CRITERIA FOR SIGNIFICANCE
Reduction of the duration of immobility during the last 4 minutes. One-way ANOVA followed by Dunnett’s test is applied for a comparison between vehicle and treated groups.
TURNAROUND TIME
STANDARD
20 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
CNS/Addiction/Pain
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.

 

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