Bone Cancer Pain, MRMT-1

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BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
Pain may be induced by intra-tibial injections of syngeneic MRMT-1 rat mammary gland carcinoma cells. Such assays are useful to detect agents with primarily mild to moderate peripheral as well as strong central analgesic activity (e.g., cyclooxygenase inhibitors and opioids, respectively).
ORGANISM
Rat
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Groups of 10 female Sprague-Dawley (SD) rats weighing 240 ± 20 grams (g) are employed. A 3 mL (3 x 10e4 cells) volume of culture medium or tumor cells in medium is slowly injected into the intramedullary cavity on Day 0 for bone cancer pain induction. The animals are pre-selected by reduction 8 g or more of force relative to the response of the individual paw before tumor cell injection on Day 18. The manual von Frey test is repeated at 1 hour (h) post test compound administration by oral gavage (PO). One-way ANOVA followed by Dunnett’s test is applied for comparison between vehicle control and test article groups. p<0.05 is considered significant. Suggested Testing: n=8/group (study design dependent). Analgesic activity assessed at an initial dose of 100 mg/kg. Dosing volume at 10 mL/kg.
CRITERIA FOR SIGNIFICANCE
Reduction of bone cancer-induced allodynia. One-way ANOVA followed by Dunnett's test is applied for comparison between vehicle control and treated groups.
TURNAROUND TIME
STANDARD
60 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
Oncology/Immuno-Oncology
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.

 

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