Bleeding Time, Mouse
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OVERVIEW
DOCUMENTS/REFERENCES
BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
Bleeding time is primarily affected by platelet function and less so by coagulation factors. When vascular injury occurs, platelets function in both primary hemostasis (platelet adhesion, secretion, aggregation) and in secondary hemostasis (coagulation). As the result of a vascular injury platelets come in contact with the subendothelium (collagen, fibronectin) and adhere to portions of it. Platelet cylooxygenase-1 (COX-1) generation of thromboxane A2 (TXA2), platelet ADP or thrombin activation of Glycoprotein IIb/IIIa as well as other stimulating agents (serotonin, epinephrine, thrombin) result in aggregation and cessation of bleeding.
ORGANISM
Mouse
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Test substance is administered orally (PO) to a group of 5 ICR male mice weighing 23 ± 3 grams (g) one hour before standardized transection of the tip (3 mm) of each tail. The mice, in holders, are then immediately suspended vertically with the distal 2 cm of each tail immersed in a test tube containing saline at 37oC. The time required for beginning a 15-second period of bleeding cessation is then determined; a maximum cut-off time of 180 seconds is used. Prolongation of bleeding time by 50 percent or more (50%) relative to a vehicle control group of animals is considered significant. One-way ANOVA followed by Dunnett's test is applied for comparison between vehicle and treatment groups. p<0.05 is considered significant. Suggested Testing: n=5/group (study design dependent). Assessed at an initial dose of 30 mg/kg. Dosing volume at 10 mL/kg.
CRITERIA FOR SIGNIFICANCE
Increase ≥50% in time required for ceasing bleeding of transected tip (3 mm) of tail immersed in saline at 37oC in a maximum cut-off time of 180 seconds. One-way ANOVA followed by Dunnett's test is ap
TURNAROUND TIME
STANDARD
20 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
Other
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.
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