Androgenetic Alopecia (AGA), Testosterone-induced, Mouse

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BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
Androgenetic alopecia (AGA) is a genetic disease that affects men and women, and it is associated with the androgen steroid hormone. Testosterone is found to induce early hair regression, hair density loss, and changes in hair morphology. Daily treatment of testosterone induces thinning of the hair coat along the upper dorsum. Testosterone-induced AGA model is commonly used to study the anti-androgenic efficacy of test articles in preventing the progression of hair loss.
ORGANISM
Mouse
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Groups of 10 male C57BL/6 mice at 7 weeks of age are used. Animals are anesthetized with isoflurane and the hair on the dorsal skin is removed by shaving an area of 2.5 × 4 cm2 with electronic clipper and hair removal cream on Day 0. For induction of AGA, the dorsal region of mouse is injected subcutaneously (SC) with 100 µL of testosterone solution (0.5 mg/mouse in corn oil) once daily (QD) from Day 1 to Day 20. Sham control mice are without any treatment. After 30 minutes of testosterone challenges, vehicle, test articles, or reference compound, Finasteride at 0.5 mg/kg will be given by oral gavage (PO) once daily (QD) from Day 1 to Day 20 for 20 days. Body weight is recorded daily. The degree of hair growth of mice is evaluated by one designated trained technician according to the scoring system during the study period. The back skin of mice is photographed twice weekly on Days 0, 3, 7, 10, 14, 17 and 21. At termination on Day 21, the mice are euthanized by CO2 asphyxiation. Dorsal skin tissue from all animals is collected and weighed. The skins can be fixed in 10% formalin for histopathological analysis to detect the total hair follicle morphology and follicle numbers (Optional).
CRITERIA FOR SIGNIFICANCE
ANOVA and Dunnett’s test are used to ascertain the difference between vehicle control and treated mice. Significance is set at p<0.05 level.
TURNAROUND TIME
STANDARD
45 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
Metabolic Diseases
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.

 

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