Allergy, Passive Cutaneous Anaphylaxis (PCA), Mouse
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DOCUMENTS/REFERENCES
BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
Passive cutaneous anaphylaxis (PCA) in mice is an animal model for type I hypersensitivity reaction. Anti-dinitrophenol (DNP) IgE antibody and DNP-human serum albumin (HSA) antigen are used to induce PCA, and this can serve as a model to evaluate anti-allergy therapeutic efficacy.
ORGANISM
Mouse
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Groups of 6 male BALB/c mice at 8 weeks old are used. Twenty-four (24) hours before antigen challenge, the animals are lightly anesthetized with isoflurane and each animal is passively sensitized by intradermal injection of anti-DNP IgE (0.08 µg/ear) to the right ear in a volume of 20 µL. Vehicle, test articles, and the reference compound, cyproheptadine, are given orally (PO) 1 hour before antigen challenge. Mice are then challenged by intravenous (IV) injection of DNP-HSA (60 µg/mouse containing 1% Evans blue) into the tail vein in a volume of 200 µL. Thirty (30) minutes after antigen challenge, ear swelling in both ears is measured by an experimenter blinded to the treatment group, with a dial thickness micrometer gauge as an index of inflammation. Percent inhibition is calculated according to the formula: (Ic – It)/Ic × 100, where Ic and It refer to the increase of ear thickness (mm) in control and treated mice, respectively. Both ears are harvested, weighed, and photographed, and ½ right ears are transferred into 250 µL formamide at 63°C for 16 h. The amount of extracted dye is measured using a spectrophotometer at 620 nm. ½ right ears are fixed in 10% formalin for toluidine blue (TB) staining (optional).
CRITERIA FOR SIGNIFICANCE
p<0.05 compared to vehicle control is considered significant activity
TURNAROUND TIME
STANDARD
20 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
Inflammation/Allergy
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.
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