Acinetobacter baumannii OXA-23 OXA-65 TEM-1B (CDC & FDA AR Isolate Bank #0294) MIC
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OVERVIEW
DOCUMENTS/REFERENCES
BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
The Minimum Inhibitory Concentration (MIC) Assay assesses the in vitro antimicrobial potency of the test article(s). The guidelines of the broth microdilution method of the Clinical and Laboratory Standards Institute are followed for the preparation of inoculum, medium and reading of the MIC value. In this assay, the MIC is the lowest concentration of an agent that completely inhibits visible growth. Antibiotic resistance of this strain, described below, is based on susceptibility studies conducted by Pharmacology Discovery Services following our standard MIC assay method. Interpretation of resistance followed the Clinical Laboratory Standards Institute (CLSI) M100 interpretive criteria guidelines. Additional antibiotic susceptibility data can be found on the CDC & FDA AR Isolate Bank website (https://wwwn.cdc.gov/arisolatebank/). Antibiotic resistance genes were annotated by the CDC & FDA AR Isolate Bank from whole genome sequence using the ResFinder database (https://wwwn.cdc.gov/arisolatebank/).
SPECIES
Acinetobacter baumannii
STRAIN COMMON NAME
AR Bank #0294
ORGANISM
Gram-negative Bacteria
INCUBATION CONDITIONS
Aerobic
STRAIN
Acinetobacter baumannii
NOTABLE ANTIBIOTIC RESISTANCE GENE
OXA-23, OXA-65, CRAB
ANTIBIOTIC RESISTANCE GENES
aac(3)-IIa,aph(3')-VIa,OXA-23,OXA-65,strA,strB,sul2,TEM-1B
ANTIBIOTIC RESISTANCE
| CLASS | ANTIBIOTIC |
| Aminoglycoside | Amikacin |
| Aminoglycoside | Gentamicin |
| Carbapenem | Imipenem |
| Carbapenem | Meropenem |
| Cephalosporin | Cefepime |
| Cephalosporin | Ceftazidime |
| Cephalosporin | Ceftriaxone |
| Fluoroquinolone | Ciprofloxacin |
| Folate Pathway Inhibitor | Trimethoprim/Sulfamethoxazole |
| β-lactam/β-lactamase Inhibitor Combination | Ampicillin/Sulbactam |
| β-lactam/β-lactamase Inhibitor Combination | Piperacillin/Tazobactam |
GENOMIC ACCESSION NUMBER
SAMN04901684
SOURCE
STRAIN SOURCE
CDC & FDA AR Isolate Bank
STRAIN SOURCE ID
AR Bank #0294
ASSAY INFORMATION
ASSAY TYPE
Functional
DETECTION METHOD
Observation / Behavioral
MEASURED RESPONSE
Score
TESTING INFORMATION
PROCEDURE SUMMARY
The test substance is dissolved in 100% DMSO (or specified vehicle) and diluted by 2-fold serial titrations for a total of 11 concentrations. A 4 µL aliquot of each dilution is added to 196 µL of broth medium seeded with the organism in a 96-well plate. The highest test substance concentration is 128 µg/mL (or specified concentration) and the final vehicle concentration is 2 percent. Following incubation, the test plate is visually examined and each well is scored for growth or complete inhibition of growth and the MIC is then recorded. Each test substance is evaluated in duplicate. Vehicle-control and an active reference agent are used as blank and positive controls, respectively. Alterations to the protocol can be accommodated with advanced notification.
INOCULUM
2 – 8 x 10⁵ CFU/mL
INCUBATION
20-24 h at 35°C ± 2°C
CONTROL INHIBITOR
Colistin (1 µg/mL)
CRITERIA FOR SIGNIFICANCE
The minimum inhibitory concentration (MIC) that prevents pathogen growth is determined using a broth assay.
TEST CONCENTRATION / DOSE
Suggested concentration for initial testing is 128 µg/ml
TEST SAMPLE REQUIREMENTS
1 mg
TURNAROUND TIME
STANDARD
15 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
Infectious Disease
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
The minimum bactericidal concentration (MBC) may be determined following the M26-A guidelines of the Clinical and Laboratory Standards Issue.
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