Neisseria gonorrhoeae in vivo panel with agar MIC
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OVERVIEW
DOCUMENTS/REFERENCES
BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
The Minimum Inhibitory Concentration (MIC) Assay assesses the in vitro antimicrobial potency of the test article(s). In this assay, the MIC is the lowest concentration of an agent that completely inhibits visible growth. The MIC protocol with Neisseria gonorrhoeae follows the Clinical and Laboratory Standards Institute (CLSI) agar dilution method. A panel of N. gonorrhoeae strains was tested (FA1090 ATCC 700825, AR Bank #0179-StrR, AR Bank #0181-StrR, and H041 NCTC 13820-StrR).
NUMBER OF ASSAYS ON PANEL
4
ASSAYS ON PANEL
| FAMILY | NAME | ITEM NUMBER | PROTEIN NAME | ORGANISM | ASSAY SUB TYPE |
| Neisseria gonorrhoeae FA1090 (ATCC 700825) Agar MIC | 612577 | Gram-negative Bacteria | |||
| Neisseria gonorrhoeae Azithromycin Non-Susceptible (CDC & FDA AR Isolate Bank #0179-StrR) Agar MIC | 612578 | Gram-negative Bacteria | |||
| Neisseria gonorrhoeae Azithromycin Non-Susceptible (CDC & FDA AR Isolate Bank #0181-StrR) Agar MIC | 612579 | Gram-negative Bacteria | |||
| Neisseria gonorrhoeae Ceftriaxone Non-Susceptible (H041 WHO-X StrR) Agar MIC | 612580 | Gram-negative Bacteria |
SPECIES
Neisseria gonorrhoeae
ORGANISM
Gram-negative Bacteria
INCUBATION CONDITIONS
Aerobic
ASSAY INFORMATION
ASSAY TYPE
Functional
DETECTION METHOD
10⁴ CFU/mL
TESTING INFORMATION
PROCEDURE SUMMARY
The agar dilution method follows the guidelines of the Clinical and Laboratory Standards Institute (CLSI), M07-A11, and thus yields data that is suitable for publication or regulatory submission. The antimicrobial test article is incorporated into an agar medium at eight concentrations varying by two-fold serial dilutions. Medium for N. gonorrhoeae is GC agar base with 1% defined growth supplement. Pathogen suspension, 0.2 µl, is spotted on the test medium surface with a replicator device yielding 1-2 x 10⁴ CFU/spot. The inoculated agar plates are incubated at 36 ± 1°C in an atmosphere of containing 5% CO₂ for 20 to 24 hours before reading the MICs. Complete, 100% visual growth inhibition is the MIC endpoint criteria. Vehicle control and an active antibiotic were used as blank and positive controls. Alterations to the protocol can be accommodated with advanced notification.
SUBSTRATE
Neisseria gonorrhoeae
ATP CONCENTRATION
GC agar + 1% isovitalex
CRITERIA FOR SIGNIFICANCE
The minimum inhibitory concentration (MIC) that prevents pathogen growth is determined using a agar-based assay.
TURNAROUND TIME
STANDARD
20 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
Infectious Disease
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
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