Inflammatory Bowel Disease (IBD), DSS-Induced Colitis, Mouse

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BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
Human inflammatory bowel disease (IBD) is a chronic inflammatory condition comprise of two major disorders, Crohn's disease and ulcerative colitis. Dextran Sulfate Sodium (DSS)-induced IBD is a chemically-induced model using DSS in the drinking water that induces ulcerative colitis-like inflammation. The fundamental symptoms of IBD are abdominal pain, bloody diarrhea and body weight loss. DSS-induced IBD is commonly used to study the mechanisms of gastrointestinal inflammation and evaluate the therapeutic efficacy.
ORGANISM
Mouse
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Groups of 6 female C57BL/6 mice or male BALB/c mice at age of the 8-9 weeks old are used. Dextran sulfate sodium (DSS), molecular weight 36,000-50,000 Da (MP Biomedicals) are used. 2.5% DSS in C57BL/6 mice or 5% DSS in BALB/c mice is given in autoclaved drinking water for 5 days, and then changed to normal drinking water for the following 5 days in the study. Mice in the sham group are given drinking water only. Test articles, vehicle (10 mL/kg, PO), and the standard, Minocycline (100 mg/kg, PO) are administered from Day 6 once daily for 4 consecutive days. During the study period, body weight, fecal occult blood, and stool consistency will be recorded from Day 6 to Day 10. On Day 10, the mice are euthanized by carbon dioxide asphyxiation and colon length is recorded and weighed.
CRITERIA FOR SIGNIFICANCE
p<0.05 compared to vehicle control is considered significant activity
TURNAROUND TIME
STANDARD
30 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
Inflammation/Allergy
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.

 

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