In Vitro Solubility Test

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BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
LabTech NEPHELOstar microplate reader. This method is a reliable technique to measure the solubility of a test agent in liquid solutions using a 96-well plate format. Laser nephelometry measures forward scattered light when a laser beam is directed through a solution. The presence of particulates in the solution results in forward-scattered light which is measured as counts.  The intensity of scattered light is linearly proportional to the concentration of particles in the solution.  The Nephelometric method has been shown to produce results equivalent to those produced by an HPLC method and to be largely unaffected by colored solutions.
ASSAY INFORMATION
ASSAY TYPE
Functional
TESTING INFORMATION
PROCEDURE SUMMARY
Compound is tested at 5 and 10 mg/mL (or two concentrations to be determined by the clients) in the following seven default formulation: (1) 5% DMSO/5% Solutol® HS-15/90% of PBS; (2) 10% Cremophor® EL/10% ethanol/80% of PBS; (3) 10% DMA/10% EtOH/20%PG /60% of PBS; (4) 10% DMA/20% PG/40% PEG-400/30% of PBS (IV with slow bolus); (5) 40% PEG-400/10% ethanol/50% of PBS (IV with slow bolus); (6) 20% PEG-400/10% Cremophor/70% of PBS; (7) 0.5% Tween 80/ 99.5% of 0.9% NaCl. Solubility is assessed by laser nephelometry in 96-well plates. Solubility data are categorized as soluble, slightly soluble and not soluble and delivered as a spreadsheet report: (1) Soluble = result is ≤ + 15 of blank; (2) Slightly Soluble = result is + 15 ~ + 25 of blank; (3) Insoluble = result is > + 25 of blank
TURNAROUND TIME
STANDARD
20 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
Other
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-1 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.

 

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