Dermatitis, Contact, Oxazolone-Induced, Chronic
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OVERVIEW
DOCUMENTS/REFERENCES
BIOLOGICAL INFORMATION
BACKGROUND INFORMATION
Allergic contact dermatitis (ACD) is a common disease caused by specific inflammatory reactions. It is a delayed-type hypersensitivity (DTH) reaction consisting of an induction (sensitization) phase and an elicitation (challenge) phase. The chronic oxazolone-induced allergic contact dermatitis mouse model is commonly used to study the mechanisms of skin inflammation and evaluate the therapeutic efficacy.
ORGANISM
Mouse
ASSAY INFORMATION
ASSAY TYPE
In Vivo
TESTING INFORMATION
PROCEDURE SUMMARY
Male BALB/c mice weighing 22 ± 2 g are randomly divided into groups of 6 each. The animals are sensitized by applying oxazolone (100 µL, 1.0% in acetone) to their pre-shaved abdominal surface (Day 0). Five days later, oxazolone (0.5%, 20 µL/ear) is applied on Day 5, and then daily from Day 7 till Day 12. On Days 7-18, vehicle and test articles are applied daily on the right ear 3 hour before oxazolone challenge (0.5%, 20 µL/ear) on Days 7-12 and dosing continues without oxazolone through Day 18. Ear swelling is measured on Day 0 and pre-dose from Day 7 through Day 19 daily. On Day 19, ear weight is determined after ear thickness is measured. Percent inhibition is calculated according to the formula: (Ic – It)/Ic x 100, where Ic and It refer to increase of ear thickness (mm) in control and treated mice, respectively. All values represent mean ± SEM in individual groups. Two-way ANOVA followed by Bonferroni test are applied for comparison between vehicle control and treated groups. Significance is considered at p<0.05.
CRITERIA FOR SIGNIFICANCE
p<0.05 compared to vehicle control is considered significant activity
TURNAROUND TIME
STANDARD
30 Days
CLINICAL RELEVANCE
THERAPEUTIC AREA
Inflammation/Allergy
ADDITIONAL INFORMATION
BRAND
PDS
TESTING LOCATION
Taiwan - Taipei
OTHER INFORMATION
We will readily accommodate client-specified alterations. These studies are performed at our AAALAC accredited BSL-2 laboratory in Taipei, Taiwan. All aspects of this work are performed in general accordance with the Guide for the Care and Use of laboratory animals (National Academy Press, Washington, DC, 2011). The study protocol was approved by the Pharmacology Discovery Services IACUC and is performed with the oversight of veterinarians to assure the humane treatment of laboratory animals.
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