In vitro Microbiology

 

Pharmacology Discovery Services provides a robust portfolio of both in vitro potency and in vivo efficacy antimicrobial services to support the discovery and development of novel antimicrobials in our BSL-2 facility.

With a comprehensive collection of more than 750 clinically relevant Gram-positive and Gram-negative bacteria including multi-drug resistant (MDR) organisms, anaerobic bacteria, yeast, and filamentous fungi, we provide in vitro antimicrobial services to help your drug development program succeed. Most strains are phenotyped and the acquired antibiotic resistance genes are genotyped. To accelerate your MIC testing, fungal, bacterial and N. gonorrhoeae pre-designed panels are also available. The minimum bactericidal concentration (MBC) may be determined following the M26-A guidelines of the Clinical and Laboratory Standards Institute.


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MIC Testing

The Minimum Inhibitory Concentration (MIC) testing from Pharmacology Discovery Services has over 750 strains of clinically relevant pathogens, including:

  • Bacteria – aerobes and anaerobes
  • Multidrug Resistant Strains (MDR) – E. coli, K. pneumoniae, N. gonorrhoeae, A. baumannii, P. aeruginosa, Enterococcus, Staphylococcus and Streptococcus
  • Fungi – yeasts, molds and dermatophytes
  • Characterization – most strains are phenotyped

To accelerate MIC testing off-the-shelf panels are available:

  • Bacterial, Fungal, N. gonorrhoeae and ESKAPE pathogens
  • Custom MIC panels - can be created with any of our available strains
  • Minimum bactericidal concentration (MBC) - may be determined following the M26-A guidelines of the Clinical and Laboratory Standards Institute

Once MIC testing is complete we have a selection of other In vitro assays to further characterize antimicrobial compounds, including:

  • Time Kill Assay – to determine if a compound has microbicidal properties
  • Checkerboard Assay – to assess antimicrobial potency of two compounds alone and in combination
  • Resistance Emergence – a group of custom assays to measure the frequency of resistance emergence
  • Membrane Lysis Assay - to detect the disruption of S. aureus cell membrane
  • Red Blood Lysis – to test if a compound can lyse human RBCs
  • Cytotoxicity Assays - to measure cytotoxicity, 40 human and rodent cell lines are available

Ordering MIC Assays

There are two options available to place an order for MIC assays. You may complete an order form and contact our client services department or you may place an order using the eCommerce system from our partner lab Eurofins Pharma Discovery Services.

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